FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes.This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. Specifications Enzyme Xbal Cutting Site 5'-T↓CTAGA-3' 3'-AGATC↑T-5' Optimal Reaction Temperature 37℃ Sensitive to Heat Inactivation YES Type lls RE NO Methylation Sensitivity Not CpG Methylation-Sensitive, Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive,Not EcoKl Methylation-Sensitive, Not EcoBI Methylation-Sensitive Star Activity 3 h incubation did not show star activity, and delayed enzyme digestion might show star activity Isoschizomers NO Components Components No. Name 15033ES76 15033-A FuniCut™ Xbal 500 μL 15033-B 10xFuniCut™ Buffer 3 x 1 mL 15033-C 10xFuniCut™ Color Buffer* 3 x 1 mL 【Note】: 10× FuniCut™ Color Buffer includes red and yellow tracking dyes, allowing direct loading of reaction products onto agarose gels without additional loading buffer. In 1% agarose gel electrophoresis, the red dye co-migrates with ~2500 bp double-stranded DNA fragments, while the yellow dye migrates similarly to ~10 bp double-stranded DNA fragments.
