UltraNuclease, also known as non-specific nuclease or broad-spectrum nuclease, is a non-specific endonuclease derived from Serratia Marcescen. It can cleave at any nucleotide within the chain, completely digesting nucleic acids into 5'-monophosphate oligonucleotides of 2-5 bases in length. UltraNuclease is capable of degrading various forms (double-stranded, single-stranded, linear, circular, native, or denatured) of DNA and RNA under a wide range of conditions (6 M Urea, 0.1 M Guanidine HCl, 0.4% Triton X-100, 0.1% SDS, 1 mM EDTA, 1 mM PMSF). It is widely used for removing nucleic acids from biological products. This product is genetically engineered and expressed in Escherichia coli (E. coli), with a purity ≥99%. It is used to reduce the viscosity of cell supernatants and cell lysates, improve protein purification efficiency and functionality studies, and effectively prevent the clumping of human peripheral blood mononuclear cells (PBMCs) in cell therapy and vaccine research. It is provided as a sterile liquid enzyme stored in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% Glycerol), appearing as a clear, colorless liquid. Features Wide range of applications: degrade all forms of DNA and RNA High purity and activity: purity ≥ 99%; specific activity ≥ 1.5×106 U/mg Specification Source E. coli Molecular Weight 26.5 kDa Isoelectric Point 6.85 Purity ≥ 99%（HPLC） Enzyme Activity 250-300 U/μL Specific Activity ≥1.5×106 U/mg protein Optimum pH 8.0 (pH range 6-10) Optimum Temperature 37°C (temperature range 0-42°C) Cofactor 1-10 mM Mg2+ Storage Buffer 20 mM Tris-HCl pH8.0，2 mM MgCl2，20 mM NaCl，50% Glycerol Unit Definition One unit (U) of enzyme activity is defined as the amount of enzyme required to cause an increase in absorbance (ΔA260) of 1.0 in 30 minutes under reaction conditions of 37°C, pH 8.0 in a total reaction volume of 2.625 mL, equivalent to the complete digestion of 37 μg of salmon sperm DNA into oligonucleotides. Components Name 20156ES25 20156ES50 20156ES60 UCF.ME™ UltraNuclease 25 KU 50 KU 100 KU Application Purification of viral vaccines, viral vectors for the vaccine, and oncolytic viruses; Protein purification-Efficient removal of contaminating DNA and RNA from proteins and other biologics; Reduction of viscosity caused by nucleic acids; Sample preparation in electrophoresis and chromatography; Prevention of cell clumping. Figures 1. High Purity Figure 1. Purity analysis of the enzyme. (A) SDSPAGE showing ≥ 99% purity; (B) HPLC profile showing ≥ 99% purity. 2. Enzymatic activity testing under different conditions Figure 2. Single‑factor comparison of Universal Nuclease activity. No significant difference was observed between this product and Supplier M under identical test conditions. 3. Application Examples
