Vero Host Cell DNA Residue Detection Kit (2G) _41307ES

Yeasen - Leading Innovation in Molecular Enzymes and Reagents Verified independent store

→

Vero Host Cell DNA Residue Detection Kit is used for the quantitative analysis of Vero host cell DNA residuce in intermediate samples, semi-finished and finished products of variou s biological products. This kit adopts Taqman fluorescent probe and the polymerase chain reaction (PCR) method, which has fg level minimum detection limit and can specifically and quickly detect the residual Vero cell DNA. The kit needs to be used together with the Residual DNA Sample Preparation Kit (Cat# 18461ES). Components No. Name 41307ES50 41307ES60 41307-A Vero qPCR Mix 0.75 mL 1.5 mL 41307-B Vero Primer&Probe Mix 200 μL 400 μL 41307-C DNA Dilution Buffer 1.8 mL×2 1.8 mL×4 41307-D Vero DNA Control（30 ng/μL） 25 μL 50 μL 41307-E IC* 50 μL 100 μL *IC：Internal control Features Strong anti-interference: Achieves 70–130% spike-and-recovery across complex matrices. High sensitivity: Lower limit of quantification (LLOQ) reaches 0.5 fg/μL. Excellent precision: Intra-assay CV < 10% and inter-assay CV < 15%. High specificity: Designed for specific detection of Vero residual DNA with no interference from other genomic DNAs. Built-in internal control (IC): Detects sample inhibition or reaction errors, minimizing false negatives. Well-validated: Method validated according to ICH Q2(R2) guidelines; full validation report available. Regulatory compliant: Verified following EP 2.6.7, JP G3, and USP requirements. Reliable quality: All enzyme components are self-manufactured and fully industrialized, ensuring stable supply. Cautions 1. Please read this manual carefully before using this reagent, and the experiment should be standardized, including sample handling, reaction system preparation and sample addition. 2. Adding samples and preparing solutions is best done on ice. 3. Ensure that each component is fully vortexed and centrifuged at low speed before use. 4. For your safety and health, please wear lab coats and disposable gloves for operation. 5. This product is for research use ONLY! Applicable instrument models Include but not limited to: Bio-Rad: CFX96 Optic Module. Thermo Scientific: ABI 7500; ABI Quant Studio 5; ABI Step OnePlus. Figures 1.Good linearity: Linear range from 3 fg/μL ~ 300 pg/μL, R²= 0.999, Eff=95.6%, CV Show more