VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis. Specifications Synonyms CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73 Uniprot No. P35918-1 Source Recombinant Mouse VEGF R2/KDR Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Ala20-Glu762. Source The protein has a predicted MW of 84.11 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The products are shipped with ice pack and can be stored at -20℃ for 1 year from date of receipt. 2-7 days, 2 to 8 °C under sterile conditions after reconstitution.3-6 months, -20 to -80 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. Note 1. For your safety and health, please wear lab coats and disposable gloves for operation. 2. For research use only! Product Data
