Biotinylated Human CD155/PVR Protein (Primary Amine Labeling), hFc Tag

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Specifications Source Biotinylated Human CD155/PVR Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the C-terminal. It contains Trp21-Asn343.[Accession | P 15151-1] Molecular Weight The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGE and HPLC Activity ELISA Data: Serial dilutions of Anti-TIGIT Antibody were added into Human TIGIT, His Tag : Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.46μg/ml. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only. Show more