Recombinant Human IL-25/IL-17E Protein, His Tag _ 94118ES

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Specifications Source Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-terminal. It contains Tyr33-Gly177.[Uniprot | Q9H293-1] Molecular Weight 大小 （Molecular Weight） The protein has a predicted MW of 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Activity SPR Data: Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay Purity > 95% as determined by Tris-Bis PAGE and HPLC Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only. Show more