Product Properties Synonyms PC9; HCHOLA3; FH3; NARC-1 Source Mouse PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln35-Gln694.[Accession | Q80W65] Molecular Weight Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human LDLR, His Tag immobilized on CM5 Chip can bind Mouse PCSK9, His Tag with an affinity constant of 0.61 nM as determined in SPR assay . Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCL, 200mM L-Aginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
