Product Properties Alternative Names HSP 27,HSP28,HSPB1,Hsp27 Species Human Expression Sequence Met1-Lys205 Accession NP_001531.1 Tag C-His Expression System E.coli Molecular Weight The protein has a predicted MW of 23.74 kDa. The protein migrates to 25-30 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS, 2mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
