Product Properties Alternative Names IL-23-A,IL23p19,IL-23A,IL-23 P19 Species Human Expression Sequence Arg 20 - Pro 189 with hIgG1 Fc Tag at the C-Terminus Accession Q9NPF7 Tag Human IgG1 Fc Expression System HEK293 Molecular Weight The protein has a predicted MW of 45.43 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Activity Immobilized IL-23A(20-189) Fc Chimera Protein, Human at 1.0μg/mL (100μL/well) can bind Biotinylated Guselkumab with EC50 of 10.77-14.25 ug/mL. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Figure SDS- PAGE Loading: 2μg (R: reducing , N: non-reducing). ELISAImmobilized IL-23A(20-189) Fc Chimera Protein, Human at 1.0μg/mL (100μL/well) can bind Biotinylated Guselkumab with EC50 of 10.77-14.25 ug/mL. Storage Store at -20 ~ -80℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.