Product Properties Alternative Names Plasma kallikrein, PKK, KLK3, KLKB1, Fletcher factor, Kininogenin Species Cynomolgus Expression Sequence Gly20-Ala638 Accession A0A2K5VTJ9 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 70.19 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】Immobilized Anti-KLKB1 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus KLKB1, His Tag with the EC50 of 1.1μg/ml determined by ELISA. Reconstitution Method Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
