Product Properties Alternative Names Osteopontin, Bone sialoprotein 1, Nephropontin, SPP-1, BNSP, OPN, SPP1, Uropontin, BSP-1, ETA-1, BSPI Species Cynomolgus Expression Sequence Leu17-Asn300 Accession A0A2K5WL90 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 33.16 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
