Product Properties Alternative Names CD340, EGFR2, ErbB2, HER2, HER-2, herstatin, MLN 19, MLN19, NGL, TKR1, ERBB2, NEU, ENV, ENVW, ERVWE1, HERV7Q, HERVW Species Mouse Expression Sequence Thr23-Thr653 Accession P70424 Tag N-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 70.74 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Mouse Her2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HER2 Antibody, Rabbit IgG Tag with the EC50 of 33.9ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.