Tko RNA Helicase is derived from the TK0306 gene of the thermophilic archaeon Thermococcus kodakaraensis (strain KOD1) and belongs to the DEAD-box RNA helicase family. This enzyme is ATP-dependent and specifically unwinds single-stranded stem-loop RNA structures. It exhibits weak activity towards double-stranded RNA (dsRNA) and single-stranded DNA (ssDNA) substrates and does not act on dsRNA substrates with 5' or 3' overhangs. With an optimal temperature of 50°C, this enzyme serves as a useful tool for specifically unwinding RNA stem-loop structures under mild temperature conditions, making it suitable for applications such as RNA structural analysis and post-transcriptional regulation research. Specifications Cat.No. 14619ES08 / 14619ES20 Size 5 μg/ 20 μg Reaction Temperature 50℃ Inactivation Conditions Incubate at 80°C for 5 minutes Components Components No. Name 14619ES08 14619ES20 14619-A Tko RNA Helicase (100 µg/mL) 50 μL 200 μL 14619-B 10×Tko Reaction Buffer 1 mL 1.5 mL Instructions 1. Prepare the following reaction mixture on ice Component Volume(μL） 10×Tko Reaction Buffer 2 ssRNA (5–10 nM) 1 Tko RNA Helicase (100 µg/mL) 0.5–1 Murine RNase inhibitor(40 U/μL) 0.1 DEPC H2O Up to 20 μL 2. Incubate at 50°C for 30 min. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves, for your safety. 3. The reaction buffer for this enzyme requires 1–2 mM ATP or dATP and 2 mM Mg²⁺. The 10×Tko Reaction Buffer already contains ATP and Mg²⁺. 4. For different substrates, the enzyme amount should be adjusted; gradient testing is recommended.
