Firefly luciferase facilitates the conversion of luciferin to oxyluciferin in conditions where ATP, magnesium ions, and oxygen are present, resulting in the emission of bioluminescence at a wavelength of about 560 nm, detectable with a luminometer. CleanRNA™ Firefly Luciferase-mRNA (low dsRNA) is an mRNA is an mRNA that has been modified with a 5' cap1, a 3' poly(A) tail, and N1-Methyl-Pseudouridine Triphosphate (N1-Me-Pseudo UTP). The product is created through an in vitro transcription process using CleaScrip™ T7 RNA polymerase, which generates ultralow dsRNA content in the mRNA solution, thereby improving the in vivo performance of the Firefly Luciferase-mRNA. Specifications Catalog Number N120011S/N120011M Specifications 0.5 mg/1 mg mRNA length 2078 nt Concentration 1 mg/mL Capping efficiency 100% dsRNA ＜0.1 ng/μg Storage buffer 1 mM sodium citrate Components Component Number Component Name Storage Temperature N120011S N120011M N120011 CleanRNA™ Firefly Luciferase-mRNA (low dsRNA) -80°C 0.5 mg 1 mg Figures Integrity tested by CE (capillary electrophoresis) Capping efficiency tested by MS Instructions 1. Selection of transfection reagents. 2. Suitable for mRNA delivery, localization, and translation. 3. Applicable for analyses such as protein expression, cell activity, and in vivo imaging. Notes 1. Upon arrival, immediately store the samples at -80°C and avoid repeated freeze-thaw. 2. For the first use, be sure to wear disposable gloves, dissolve the samples on ice, and use RNase-free reagents and consumables to avoid contamination. 3. After the initial dissolution of the samples, they can be gently centrifuged and then aliquoted, avoiding repeated freezing and thawing.
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