Firefly Luciferase saRNA is usually used as an internal reference RNA to study the expression of targeted RNAs. Firefly Luciferase (Firefly luc) expresses a luciferase protein originally extracted from fireflies. This enzyme, dependent on ATP, catalyzes the oxidation of D-luciferin, producing chemiluminescence at approximately 560 nm wavelength. Firefly luc is a commonly used bioluminescent reporter gene for gene regulation and functional studies, emitting bioluminescence in the presence of the substrate luciferin. Firefly luc saRNA is synthesized and purified in a single step from a linear template via T7 transcriptase and the LZCap AU cap analog. This mRNA, upon entering cells, expresses the luciferase protein. Compared to the Cap0 structure and other commercially available Cap1 structures, the LZCap AU Cap1 structure enables more efficient and sustained RNA expression. LZCap AU Firefly luc saRNA stock solution is suitable for experiments involving RNA delivery, translation efficiency, transfection efficiency, and in vivo imaging. Specifications saRNA Length 9,388 nucleotides (including Cap1 AU structure) Concentration 1 μg/μL Specifications 100 µL, 1 mL Purity ≥90% HPLC Instructions 1. Selection of transfection reagents. 2. Suitable for mRNA delivery, localization, and translation. 3. Applicable for analyses such as protein expression, cell activity, and in vivo imaging. Notes 1. Upon arrival, immediately store the samples at -80°C and avoid repeated freeze-thaw. 2. For the first use, be sure to wear disposable gloves, dissolve the samples on ice, and use RNase-free reagents and consumables to avoid contamination.
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